Advanced CUBIC Protocols for Whole-Brain and Whole-Body Clearing and Imaging

Etsuo A Susaki, Kazuki Tainaka, Dimitri Perrin, Hiroko Yukinaga, Akihiro Kuno and Hiroki R Ueda: Advanced CUBIC Protocols for Whole-Brain and Whole-Body Clearing and Imaging. Nature protocols 2015, 10: 1709-1727.

This protocol describes in detail how to perform advanced CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis). The CUBIC protocol enables simple and efficient organ clearing, rapid imaging by light-sheet microscopy and quantitative imaging analysis of multiple samples. These protocols provide a platform for organism-level systems biology by comprehensively detecting cells in a whole organ or body.

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