kgriffiths

About Kelly Griffiths

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So far Kelly Griffiths has created 18 entries.

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Video Article Published on JoVE

Rothschild DE, Srinivasan T, Aponte-Santiago LA, Shen X, Allen IC : JoVE 111, (2016) doi:10.3791/54033, Published 18 May 2016

This protocol has been generated to outline a simple procedure to culture and treat small intestinal organoids with microbial pathogens and pathogen associated molecular patterns (PAMPs). It also emphasizes the normalization techniques […]

Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments

Rafael Di Marco Barros, Natalie A. Roberts, Robin J. Dart, Pierre Vantourout, Anett Jandke,
Oliver Nussbaumer, Livija Deban, Sara Cipolat, Rosie Hart, Maria Luisa Iannitto, Adam Laing,
Bradley Spencer-Dene, Philip East, Deena Gibbons, Peter M. Irving, Pablo Pereira, Ulrich Steinhoff,
and Adrian Hayday

A conserved biology emerges linking mucosal and skin intraepithelial lymphocyte (IEL) biology and mucosal IEL […]

Sequencing and cloning of antigen-specific antibodies from mouse memory B cells

Published in Nature Protocols

Lotta von Boehmer, Cassie Liu, Sarah Ackerman, Alexander D Gitlin, Qiao Wang, Anna Gazumyan & Michel C Nussenzweig :Nature Protocols 11, 1908-1923 (2016) doi:10.1038/nprot.2016.102, Published online 15 September 2016

Methods to identify genes encoding immunoglobulin heavy and light chains from single B lymphocytes vary in efficiency, error rate and practicability. Here we describe […]

High-resolution 3D analysis of mouse small-intestinal stroma

Published in Nature Protocols

Jeremiah Bernier-Latmani & Tatiana V Petrova :Nature Protocols 11, 1617–1629 (2016) doi:10.1038/nprot.2016.092, Published online 11 August 2016

Here we detail a protocol for whole-mount immunostaining of mouse small-intestinal villi that can be used to generate high-resolution 3D images of all gut cell types, including blood and lymphatic vessel cells, neurons, smooth muscle […]

IL-33 regulates the IgA-microbiota axis to restrain IL-1α–dependent colitis and tumorigenesis

Ankit Malik, Deepika Sharma, Qifan Zhu, Rajendra Karki, Clifford S. Guy, Peter Vogel, and Thirumala-Devi Kanneganti

Inflammatory bowel diseases (IBD) affect over 5 million individuals in the industrialized world, with an increasing incidence rate worldwide. IBD also predisposes affected individuals to development of colorectal cancer, which is a leading cause of cancer-related deaths in adults. […]

Highly Multiplexed Simultaneous Detection of RNAs and Proteins

Highly multiplexed simultaneous detection of RNAs and proteins in single cells

Andreas P Frei, Felice-Alessio Bava, Eli R Zunder, Elena W Y Hsieh, Shih-Yu Chen, Garry P Nolan & Pier Federico Gherardini: Nature Methods 2016 Jan; 269-275. Published online 2016 January 25. doi:10.1038/nmeth.3742.

This method combines the Flow cytometry possibility to acquire single cell expression […]

By |November 30th, 2016|Categories: Laboratory Techniques, Protocols||0 Comments

Lymphoid Aggregates Remodel Lymphatic Collecting Vessels that Serve Mesenteric Lymph Notes in Crohn Disease

Gwendalyn J. Randolph, Shashi Bala, Jean-François Rahier, Michael W. Johnson, Peter L. Wang, ILKe Nalbantoglu, Laurent Dubuquoy, Amélie Chau, Benjamin Pariente, Alex Kartheuser, Bernd H. Zinselmeyer, and Jean-Frederic Colombel

Early pathological descriptions of Crohn disease (CD) argued for a potential defect in lymph transport; however, this concept has not been thoroughly investigated. In mice, poor […]

By |November 23rd, 2016|Categories: Featured Papers, November 2016||0 Comments

Human Norovirus Culture in B Cells

Human norovirus culture in B cells

Melissa K Jones, Katrina R Grau, Veronica Costantini, Abimbola O Kolawole, Miranda de Graaf, Pamela Freiden, Christina L Graves, Marion Koopmans, Shannon M Wallet, Scott A Tibbetts, Stacey Schultz-Cherry, Christiane E Wobus, Jan Vinjé & Stephanie M Karst: Nat Protoc. 2015 Dec; 10(12): 1939-47. Published online 2015 Oct 29. […]

By |November 23rd, 2016|Categories: Protocols, Publications||0 Comments