SMI

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So far SMI has created 163 entries.

Murine Model of DSS-induced Acute Colitis

Murine Model of DSS-induced Acute Colitis

J Vis Exp. 2012 Feb 1;(60). pii: 3678. doi: 10.3791/3678. Investigating intestinal inflammation in DSS-induced model of IBD. Kim JJ1, Shajib MS, Manocha MM, Khan WI. 

Inflammatory bowel disease (IBD) encompasses a range of intestinal pathologies, the most common of which are ulcerative colitis (UC) and Crohn’s Disease (CD). Both UC […]

By |February 27th, 2017|Categories: Gastrointestinal System, Protocols, Publications||0 Comments

Unsupervised High-Dimensional Analysis Aligns Dendritic Cells across Tissues and Species

By Guilliams M, Dutertre CA, Scott CL, McGovern N, Sichien D, Chakarov S, Van Gassen S, Chen J, Poidinger M, De Prijck S, Tavernier SJ, Low I, Irac SE, Mattar CN, Sumatoh HR, Low G, Chung J, Chan D, Tan KK, Tony Lim KH, Fossum E, Bogen B, Choolani M, Chan J, Larbi A, […]

Airway Hyperresponsiveness in Murine Tracheal Rings

Assessment of Airway Hyperresponsiveness in Murine Tracheal Rings. Methods Mol Biol. 2013;1032:257-69. doi: 10.1007/978-1-62703-496-8_20.

Isolated tracheal rings have often been used to directly measure the contractile output of airway smooth muscle (ASM). Here, we describe the method for excising murine tracheas, mounting tracheal rings in organ baths, and measuring the isometric forces generated by the ASM when […]

By |February 12th, 2017|Categories: Protocols, Respiratory System||0 Comments

Secretory leukoprotease inhibitor is required for efficient quercetin-mediated suppression of TNFα secretion

Stefania De Santis, Dale Kunde, Grazia Serino, Vanessa Galleggiante, Maria Lucia Caruso, Mauro Mastronardi, Elisabetta Cavalcanti, Nicole Ranson, Aldo Pinto, Pietro Campiglia, Angelo Santino, Rajaraman Eri, Marcello Chieppa

Our data encourages the development of nutritional strategies to improve the efficiency of current therapies for intestinal chronic inflammatory syndrome and reduce the risks of colorectal cancer […]

By |January 19th, 2017|Categories: Featured Papers, January 2017|Tags: , , , , |0 Comments

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Video Article Published on JoVE

Rothschild DE, Srinivasan T, Aponte-Santiago LA, Shen X, Allen IC : JoVE 111, (2016) doi:10.3791/54033, Published 18 May 2016

This protocol has been generated to outline a simple procedure to culture and treat small intestinal organoids with microbial pathogens and pathogen associated molecular patterns (PAMPs). It also emphasizes the normalization techniques […]

By |January 11th, 2017|Categories: Ex Vivo, Laboratory Techniques, Protocols||0 Comments

Epithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments

Rafael Di Marco Barros, Natalie A. Roberts, Robin J. Dart, Pierre Vantourout, Anett Jandke,
Oliver Nussbaumer, Livija Deban, Sara Cipolat, Rosie Hart, Maria Luisa Iannitto, Adam Laing,
Bradley Spencer-Dene, Philip East, Deena Gibbons, Peter M. Irving, Pablo Pereira, Ulrich Steinhoff,
and Adrian Hayday

A conserved biology emerges linking mucosal and skin intraepithelial lymphocyte (IEL) biology and mucosal IEL […]

Sequencing and cloning of antigen-specific antibodies from mouse memory B cells

Published in Nature Protocols

Lotta von Boehmer, Cassie Liu, Sarah Ackerman, Alexander D Gitlin, Qiao Wang, Anna Gazumyan & Michel C Nussenzweig :Nature Protocols 11, 1908-1923 (2016) doi:10.1038/nprot.2016.102, Published online 15 September 2016

Methods to identify genes encoding immunoglobulin heavy and light chains from single B lymphocytes vary in efficiency, error rate and practicability. Here we describe […]

High-resolution 3D analysis of mouse small-intestinal stroma

Published in Nature Protocols

Jeremiah Bernier-Latmani & Tatiana V Petrova :Nature Protocols 11, 1617–1629 (2016) doi:10.1038/nprot.2016.092, Published online 11 August 2016

Here we detail a protocol for whole-mount immunostaining of mouse small-intestinal villi that can be used to generate high-resolution 3D images of all gut cell types, including blood and lymphatic vessel cells, neurons, smooth muscle […]

IL-33 regulates the IgA-microbiota axis to restrain IL-1α–dependent colitis and tumorigenesis

Ankit Malik, Deepika Sharma, Qifan Zhu, Rajendra Karki, Clifford S. Guy, Peter Vogel, and Thirumala-Devi Kanneganti

Inflammatory bowel diseases (IBD) affect over 5 million individuals in the industrialized world, with an increasing incidence rate worldwide. IBD also predisposes affected individuals to development of colorectal cancer, which is a leading cause of cancer-related deaths in adults. […]

Highly Multiplexed Simultaneous Detection of RNAs and Proteins

Highly multiplexed simultaneous detection of RNAs and proteins in single cells

Andreas P Frei, Felice-Alessio Bava, Eli R Zunder, Elena W Y Hsieh, Shih-Yu Chen, Garry P Nolan & Pier Federico Gherardini: Nature Methods 2016 Jan; 269-275. Published online 2016 January 25. doi:10.1038/nmeth.3742.

This method combines the Flow cytometry possibility to acquire single cell expression […]

By |November 30th, 2016|Categories: Laboratory Techniques, Protocols||0 Comments