Applications of Mouse Airway Epithelial Cell Culture for Asthma Research

Applications of Mouse Airway Epithelial Cell Culture for Asthma Research. Horani A, Dickinson JD, Brody SL. Methods Mol Biol. 2013;1032:91-107. doi: 10.1007/978-1-62703-496-8_7.

Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell–cell interactions, and the role of immune system factors in asthma pathogenesis. In this chapter, we discuss the application of mouse tracheal […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols||0 Comments

Interactions of Intestinal Epithelial Cells

Interactions of Intestinal Epithelial Cells with Bacteria and Immune Cells: Methods to Characterize Microflora and Functional Consequences. Canny G, Swidsinski A, McCormick BA. Methods Mol Biol. 2006;341:17-35.

Epithelial cells at all mucosal surfaces are potentially apposed to bacteria, particularly in the intestine. It is established that intestinal epithelial cells (IECs) represent an important barrier between […]

By |May 29th, 2014|Categories: Ex Vivo, Protocols||0 Comments

Natural Killer Cells in Human Pregnancy

Male V, Trundley A, Gardner L, Northfield J, Chang C, Apps R, Moffett A.  Methods Mol Biol. 2010;612:447-63. doi: 10.1007/978-1-60761-362-6_30.

Natural killer (NK) cells account for 70% of the leukocytes in the mucosal lining of the uterus (the decidua) in the first trimester of pregnancy. They are CD56(superbright) granulated cells expressing a repertoire of Killer-cell […]

By |May 29th, 2014|Categories: Ex Vivo, Protocols||0 Comments

In Vitro Culture and Expansion of Human Limbal Epithelial Cells

Mariappan I, Maddileti S, Savy S, Tiwari S, Gaddipati S, Fatima A, Sangwan VS, Balasubramanian D, Vemuganti GK. Nat Protoc. 2010 Aug;5(8):1470-9. doi: 10.1038/nprot.2010.115. Epub 2010 Jul 29.

Limbal stem cells (LSCs) have an important role in the maintenance of the corneal surface epithelium, and autologous cultured limbal epithelial cell transplantations have contributed substantially to […]

By |May 29th, 2014|Categories: Ex Vivo, Protocols||0 Comments

Characterization of Mouse and Human Esophageal Epithelial Cells

Isolation and Characterization of Mouse and Human Esophageal Epithelial Cells in 3D Organotypic Culture. Kalabis J, Wong GS, Vega ME, Natsuizaka M, Robertson ES, Herlyn M, Nakagawa H, Rustgi AK. Nat Protoc. 2012 Jan 12;7(2):235-46. doi: 10.1038/nprot.2011.437.

This protocol describes the isolation and characterization of mouse and human esophageal epithelial cells and the application of […]

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Isolation of CD4+ T Cells from Murine Lungs

Isolation of CD4+ T Cells from Murine Lungs: A Method to Analyze Ongoing Immune Responses in the Lung. Sauer KA, Scholtes P, Karwot R, Finotto S. Nat Protoc. 2006;1(6):2870-5.

The regulation of the cellular immune response in lung diseases is not yet fully understood. Isolating different subsets of immune cells directly from the lung is […]

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Use of Human Tissue Explants to Study Human Infectious Agents

Grivel JC, Margolis L. Nat Protoc. 2009;4(2):256-69. doi: 10.1038/nprot.2008.245.

The study of human cell-cell and cell-pathogen interactions that occur in the context of complex tissue cytoarchitecture is critical for deciphering the mechanisms of many normal and pathogenic processes. This protocol describes methods for culturing and infecting explants of human tissues to study the pathogenesis of […]

By |May 29th, 2014|Categories: Ex Vivo, Protocols||0 Comments

Models of Epithelial Candida

Models of Oral and Vaginal Candidiasis Based on in vitro Reconstituted Human Epithelial. Martin Schaller, Katherina Zakikhany, Julian R Naglik, Günther Weindl & Bernhard Hube Nature Protocols 1, 2767-2773 doi:10.1038/nprot.2006.4

This protocol describes the setup, maintenance and characteristics of models of epithelial Candida infections based on well-established three-dimensional organotypic tissues of human oral and vaginal […]

By |May 27th, 2014|Categories: Ex Vivo, Protocols||0 Comments