A Human Intestinal M-Cell-Like Model for Investigating Particle, Antigen and Microorganism Translocation

A Human Intestinal M-Cell-Like Model for Investigating Particle, Antigen and Microorganism Translocation. Beloqui, et al., Nature Protocols, June 15, 2017

The specialized microfold cells (M cells) in the follicle-associated epithelium of intestinal Peyer’s patches serve as antigen-sampling cells of the intestinal innate immune system. Unlike ‘classical’ enterocytes, they are able to translocate diverse particulates without […]

By |November 20th, 2017|Categories: Gastrointestinal System, Protocols||0 Comments

Constructing Cellular Niche Properties by Localized Presentation of Wnt Proteins on Synthetic Surfaces

Lowndes, et al., Nature Protocols, June 29, 2017

This protocol describes a detailed protocol based on covalent binding of nucleophilic groups on Wnt proteins to activated carboxylic acid or glutaraldehyde groups functionalized on synthetic surfaces. This method can be used to covalently immobilize Wnt3a proteins on microbeads or a glass surface, which can help in […]

By |November 13th, 2017|Categories: Gastrointestinal System, Protocols||0 Comments

Murine Model of DSS-induced Acute Colitis

Murine Model of DSS-induced Acute Colitis

J Vis Exp. 2012 Feb 1;(60). pii: 3678. doi: 10.3791/3678. Investigating intestinal inflammation in DSS-induced model of IBD. Kim JJ1, Shajib MS, Manocha MM, Khan WI. 

Inflammatory bowel disease (IBD) encompasses a range of intestinal pathologies, the most common of which are ulcerative colitis (UC) and Crohn’s Disease (CD). Both UC […]

By |February 27th, 2017|Categories: Gastrointestinal System, Protocols, Publications||0 Comments

In vivo Imaging and Tracking of Host-Microbiota Interactions via Metabolic

In vivo Imaging and Tracking of Host-Microbiota Interactions via Metabolic Labeling of Gut Anaerobic Bacteria
Naama Geva-Zatorsky, David Alvarez, Jason E. Hudak, Nicola C. Reading, Deniz Erturk-Hasdemir, Suryasarathi Dasgupta, Ulrich H. von Andrian, and Dennis L. Kasper: Nat Med. 2015 Sep; 21(9): 1091–1100. Published online 2015 Aug 17. doi:  10.1038/nm.3929

“The intestine is densely populated by anaerobic […]

T Lymphocyte Density and Distribution in Human Colorectal Mucosa, and Inefficiency of Current Cell Isolation Protocols

T Lymphocyte Density and Distribution in Human Colorectal Mucosa, and Inefficiency of Current Cell Isolation Protocols
Preza GC, Yang OO, Elliott J, Anton PA, Ochoa MT: T Lymphocyte Density and Distribution in Human Colorectal Mucosa, and Inefficiency of Current Cell Isolation Protocols. PLoS One 2015 Apr 9;10(4):e0122723.

“Mucosal tissues are critical immune effector sites containing complex populations of […]

Isolation and Cytokine Analysis of Lamina Propria Lymphocytes

Isolation and Cytokine Analysis of Lamina Propria Lymphocytes from Mucosal Biopsies of the Human Colon
Bowcutt R, Malter LB, Chen LA, Wolff MJ, Robertson I, Rifkin DB, Poles M, Cho I, Loke P: Isolation and Cytokine Analysis of Lamina Propria Lymphocytes from Mucosal Biopsies of the Human Colon. J Immunol Methods 2015 Jun;421:27-35. 

“Much of our understanding of […]

Analysis of Murine Lamina Propria Mononuclear Cells

Isolation and Subsequent Analysis of Murine Lamina Propria Mononuclear Cells from Colonic Tissue

Weigmann B, Tubbe I, Seidel D, Nicolaev A, Becker C, Neurath MF: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue. Nature protocols 2007, 2(10):2307-2311.

Studies on colonic cells in the lamina propria (LP) of mine are important for […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications||0 Comments

Optimization of Murine Small Intestine Leukocyte Isolation

Optimization of Murine Small Intestine Leukocyte Isolation for Global Immune Phenotype Analysis

Andrew W. Goodyear, Ajay Kumar, Steven Dow, Elizabeth P. Ryan. Optimization of murine small intestine leukocyte isolation for global immune phenotype analysis. Journal of Immunological Methods 405 (2014) 97–108.

New efforts to understand complex interactions between diet, gut microbiota, and intestinal immunity emphasize the […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications||0 Comments

Immunological Changes in IL-10-Deficient Mice

New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa

Gomes-Santos AC, Moreira TG, Castro-Junior AB, Horta BC, Lemos L, Cruz DN, Guimarães MA, Cara DC, McCafferty DM, Faria AM. New insights into the immunological changes in IL-10-deficient mice during the course of spontaneous inflammation in […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications||0 Comments

Oral Delivery of Protein Complement Inhibitor

A Novel Protocol Allowing Oral Delivery of a Protein Complement Inhibitor that Subsequently Targets to Inflamed Colon Mucosa and Ameliorates Murine Colitis

Elvington, M; Blichmann, P; Qiao, F; Scheiber, M; Wadsworth, C; et al. A novel protocol allowing oral delivery of a protein complement inhibitor that subsequently targets to inflamed colon mucosa and ameliorates murine […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications||0 Comments