Confocal Laser Endomicroscopy for in vivo Imaging

Confocal Laser Endomicroscopy and Narrow-band Imaging-aided Endoscopy for in vivo Imaging of Colitis and Colon Cancer in Mice

Waldner MJ, Wirtz S, Neufert C, Becker C, Neurath MF: Confocal laser endomicroscopy and narrow-band imaging-aided endoscopy for in vivo imaging of colitis and colon cancer in mice. Nature protocols 2011, 6(9):1471-1481.

New endoscopic techniques such as narrow-band […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications||0 Comments

Compression-Based Distance (CBD): Microbiota Composition

Fang, Y., Chia, N., White, B., Schook, L. “Compression-based Distance (CBD): A Simple, Rapid, and Accurate Method for Microbiota Composition Comparison.” BMC Bioinformatics. BMC Bioinformatics, 23 Apr. 2013.

Perturbations in intestinal microbiota composition have been associated with a variety of gastrointestinal tract-related diseases. The alleviation of symptoms has been achieved using treatments that alter the gastrointestinal tract […]

By |July 25th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments

Incubation of Precision-Cut Liver and Intestinal Slices

Preparation and Incubation of Precision-cut Liver and Intestinal Slices for Application in Drug Metabolism. de Graaf IA, Olinga P, de Jager MH, Merema MT, de Kanter R, van de Kerkhof EG, Groothuis GM.  Nat Protoc. 2010 Sep;5(9):1540-51. doi: 10.1038/nprot.2010.111. Epub 2010 Aug 19.

Precision-cut tissue slices (PCTS) are viable ex vivo explants of tissue with a […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments

Methods for Generating and Colonizing Gnotobiotic Zebrafish

Pham LN, Kanther M, Semova I, Rawls JF.  Nat Protoc. 2008;3(12):1862-75. doi: 10.1038/nprot.2008.186.

Vertebrates are colonized at birth by complex and dynamic communities of microorganisms that can contribute significantly to host health and disease. The ability to raise animals in the absence of microorganisms has been a powerful tool for elucidating the relationships between animal […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments

Chemically Induced Mouse Models of Intestinal Inflammation

Wirtz S, Neufert C, Weigmann B, Neurath MF.  Nat Protoc. 2007;2(3):541-6.

Animal models of intestinal inflammation are indispensable for our understanding of the pathogenesis of Crohn’s disease and ulcerative colitis, the two major forms of inflammatory bowel disease in humans. Here, we provide protocols for establishing murine 2,4,6-trinitro benzene sulfonic acid (TNBS)-, oxazolone- and both […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments

High Resolution Colonoscopy in Live Mice

Becker C, Fantini MC, Neurath MF. Nat Protoc. 2006;1(6):2900-4.

Endoscopy in humans is a powerful method for physicians to examine the gut for inflammatory or neoplastic changes. In medical and immunological research, animal models of intestinal diseases are established key tools to investigate the mucosal immune system, colitis and cancer development in the gut. Moreover, […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments

Isolating, Immunophenotyping and ex vivo Stimulation

Isolating, Immunophenotyping and ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes during Murine Helicobacter pylori Infection. Victoria E. Ruiz, Monisha Sachdev, Songhua Zhang, Sicheng Wen, Steven F. Moss.  Journal of Immunological Methods, Volume 384, Issues 1–2, 31 October 2012, Pages 157-163

Helicobacter pylori infection is associated with severe chronic inflammation, yet the host immune response is rarely […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments

Niche-Independent High-Purity Cultures of Lgr5+

Niche-Independent High-Purity Cultures of Lgr5+ Intestinal Stem Cells and their Progeny. Yin X, Farin HF, van Es JH, Clevers H, Langer R, Karp JM. Nat Methods. 2014 Jan;11(1):106-12. doi: 10.1038/nmeth.2737. Epub 2013 Dec 1.

Although Lgr5+ intestinal stem cells have been expanded in vitro as organoids, homogeneous culture of these cells has not been possible thus […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments

Bacterial Mediated Gastrointestinal Inflammation

Uronis J, Sun X. Methods Mol Biol. 2013;1031:197-202. doi: 10.1007/978-1-62703-481-4_22.

Mouse models have proven to be a key approach in our understanding of the etiology and physiology underlying bacterial mediated gastrointestinal infl ammation. Generally, these models are based on the inoculation of genetically susceptible mice with either commensal or pathogenic bacteria to elicit an inflammatory response. Here, we […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments

An Inducible Mouse Model of Colon Carcinogenesis

An Inducible Mouse Model of Colon Carcinogenesis for the Analysis of Sporadic and Inflammation-Driven Tumor Progression. Neufert C, Becker C, Neurath MF. Nat Protoc. 2007;2(8):1998-2004.

Colorectal cancer is a life-threatening disease that can develop spontaneously or as a complication of inflammatory bowel diseases. Mouse models are essential tools for the preclinical testing of novel therapeutic […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols||0 Comments