An Overview of the Model Organism: C. Elegans

JoVE Science Education Database. Model Organisms I: yeast, Drosophila and C. elegans. An Overview of the Model Organism: C. elegans. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5103 (2014).

Caenorhabditis elegans is a microscopic, soil-dwelling roundworm that has been powerfully used as a model organism since the early 1970″s. It was initially proposed as a […]

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Bacterial Transformation: Electroporation

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Bacterial Transformation: Electroporation. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5060 (2014).

The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores […]

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DNA Ligation Reactions

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. DNA Ligation Reactions. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5069 (2014).

Overview
Ligation can be defined as the act of joining, and in biology the term refers to an enzymatic reaction that joins two biomolecules with a covalent bond. This video describes the application […]

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Gel Purification

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Gel Purification. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5063 (2014).

Overview
Gel-purification is a standard procedure performed to recover desired DNA fragments from agarose gels after electrophoretic separation. After dissolving the gel fragment and running it through a specialized filter, this procedure yields DNA […]

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Histological Sample Preparation for Light Microscopy

JoVE Science Education Database. General Laboratory Techniques. Histological Sample Preparation for Light Microscopy. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5039 (2014).

Histology is the study of cells and tissues, which is typically aided by the use of a light microscope. The preparation of histological samples can vary greatly based on the inherent properties of […]

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Introduction to Fluorescence Microscopy

JoVE Science Education Database. General Laboratory Techniques. Introduction to Fluorescence Microscopy. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5040 (2014).

Fluorescence microscopy is a very powerful analytical tool that combines the magnifying properties of light microscopy with visualization of fluorescence. Fluorescence is a phenomenon that involves absorbance and emission of a small range of light […]

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Introduction to Light Microscopy

JoVE Science Education Database. General Laboratory Techniques. Introduction to Light Microscopy. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5041 (2014).

The light microscope is an instrument used by researchers in many different fields to magnify specimens to as much as a thousand times their original size. In its simplest form, it is composed of a […]

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Introduction to Serological Pipettes and Pipettors

JoVE Science Education Database. General Laboratory Techniques. Introduction to Serological Pipettes and Pipettors Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5034 (2014).

The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable […]

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Introduction to the Spectrophotometer

JoVE Science Education Database. General Laboratory Techniques. Introduction to the Spectrophotometer. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5038 (2014).

The spectrophotometer is a routinely used instrument in scientific research. Spectrophotometry is the quantitative measurement of how much a chemical substance absorbs light by passing a beam of light through the sample using a spectrophotometer. […]

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Making Solutions in the Laboratory

JoVE Science Education Database. General Laboratory Techniques. Making Solutions in the Laboratory. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5030 (2014).

The ability to successfully make solutions is a basic laboratory skill performed in virtually all biological and chemical experiments. A solution is a homogenous mixture of solute dissolved in bulk liquid known as the […]

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