Molecular Cloning

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Molecular Cloning. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5074 (2014).

Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector – a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. The […]

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Passaging Cells

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Passaging Cells. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5052 (2014).

Cell lines are frequently used in biomedical experiments, as they allow rapid culture and expansion of cell types for experimental analysis. Cell lines are cultured under similar conditions when compared to freshly-isolated, or […]

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PCR: The Polymerase Chain Reaction

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. PCR: The Polymerase Chain Reaction. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5056 (2014).

The polymerase chain reaction, or PCR, is a technique used to amplify DNA through thermocycling – cyles of temperature changes at fixed time intervals. Using a thermostable DNA polymerase, PCR […]

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Plasmid Purification

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Plasmid Purification. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5062 (2014).

Overview
Plasmids are circular, extra chromosomal, DNA molecules and in molecular biology they act as carriers, or vectors, for a specific DNA fragment. Bacteria are used to replicate plasmids, so that your DNA of […]

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Restriction Enzyme Digests

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Restriction Enzyme Digests. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5070 (2014).

Restriction enzymes or endonucleases recognize and cut DNA at a specific sequence. These enzymes occur naturally in bacteria as a defense against bacteriophages – viruses that infect bacteria. Bacterial restriction enzymes cut […]

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Separating Protein with SDS-PAGE

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Separating Protein with SDS-PAGE. Journal Visualized Experiments, Cambridge, MA, doi: 10.3791/5058 (2014).

Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis , or SDS-PAGE, is a widely-used technique for separating mixtures of proteins based on their size and nothing else. SDS, an anionic detergent, is used to […]

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The ELISA Method

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. The ELISA Method. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5061 (2014).

An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an experimental sample. Detection of the target protein is made possible […]

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The Western Blot

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. The Western Blot. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5065 (2014).

Overview
Western Blotting is a powerful technique utilized by many researchers to identify the presence of specific proteins in an electrophoretically-separated sample using antibodies.

Summary
Western Blotting is used to identify the presence of specific […]

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Understanding Concentration and Measuring Volumes

JoVE Science Education Database. General Laboratory Techniques. Understanding Concentration and Measuring Volumes. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5026 (2014).

Solutions are utilized to some degree in almost all biological research applications. Therefore understanding how to measure and manipulate them is imperative to any experiment. In this video, concepts in preparing solutions are introduced. […]

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Using a Hemacytometer to Count Cells

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Using a Hemacytometer to Count Cells. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5048 (2014).

Overview
Cell counting is an important step in many cell-based assays for determining cell number and viability. In general, the goal of counting is to understand how much a sample […]

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