Isolating Mucosal Lymphocytes for Cellular Immunology Assays

Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology Assays. Shacklett BL, Critchfield JW, Lemongello D.  Methods Mol Biol. 2009;485:347-56. doi: 10.1007/978-1-59745-170-3_23.

Mucosal tissues of the gastrointestinal and genitourinary tracts serve as major portals of HIV-1 transmission, and recent literature has highlighted the important role of these tissues in pathogenesis. However, our understanding of human mucosal […]

By |June 3rd, 2014|Categories: Laboratory Techniques, Protocols||0 Comments

Practical Techniques for Detection of Toll-Like Receptor-4

Ungaro R, Abreu MT, Fukata M.  Practical Techniques for Detection of Toll-Like Receptor-4 in the Human Intestine. Methods Mol Biol. 2009;517:345-61. doi: 10.1007/978-1-59745-541-1_21.

The human intestine has evolved in the presence of a diverse array of luminal microorganisms. In order to maintain intestinal homeostasis, mucosal immune responses to theses microorganisms must be tightly regulated. The intestine […]

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Determining the Activity of Mucosal Adjuvants

Baudner BC, Giudice GD.  Methods Mol Biol. 2010;626:261-85. doi: 10.1007/978-1-60761-585-9_18.

Mucosal vaccination offers the advantage of blocking pathogens at the portal of entry, improving patient’s compliance, facilitating vaccine delivery, and decreasing the risk of unwanted spread of infectious agents via contaminated syringes.Recent advances in vaccinology have created an array of vaccine constructs that can be […]

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Quantification of mRNA Using Real-Time RT-PCR

Nolan T, Hands RE, Bustin SA.  Nat Protoc. 2006;1(3):1559-82.

The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described as a “gold” standard, it is […]

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Single Nucleotide Polymorphism (SNP) Allele Frequency DNA Pools

Lavebratt C, Sengul S. Single Nucleotide Polymorphism (SNP) Allele Frequency Estimation in DNA Pools using Pyrosequencing. Nat Protoc. 2006;1(6):2573-82.

Identifying the genetic variation underlying complex disease requires analysis of many single nucleotide polymorphisms (SNPs) in a large number of samples. Several high-throughput SNP genotyping techniques are available; however, their cost promotes the use of association screening […]

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A Rapid Protocol for Construction of Adenoviral Vectors

Jager L, Hausl MA, Rauschhuber C, Wolf NM, Kay MA, Ehrhardt A. A Rapid Protocol for Construction and Production of High-Capacity Adenoviral Vectors. Nat Protoc. 2009;4(4):547-64. doi: 10.1038/nprot.2009.

High-capacity adenoviral vectors (HC-AdVs) lacking all viral coding sequences were shown to result in long-term transgene expression and phenotypic correction in small and large animal models. It has been […]

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Laser-Capture Microdissection

Espina V, Wulfkuhle JD,Calvert VS, VanMeter A, Zhou W, Coukos G, Geho DH, Petricoin EF 3rd, Liotta LA

Deciphering the cellular and molecular interactions that drive disease within the tissue microenvironment holds promise for discovering drug targets of the future. In order to recapitulate the in vivo interactions thorough molecular analysis, one must be able […]

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An Introduction to Drosophila Melanogaster

JoVE Science Education Database. Model Organisms I: yeast, Drosophila and C. elegans. An Introduction to Drosophila melanogaster. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5082 (2014).

Drosophila melanogaster, also known as the fruit fly, is a powerful model organism widely used in biological research that has made significant contributions to the greater scientific community over […]

By |April 3rd, 2014|Categories: Laboratory Techniques, Protocols||0 Comments