Respiratory System

Harvesting Alveolar Macrophages from the Mouse

Harvesting Alveolar Macrophages from the Mouse Using Lung Lavage

Chavez-Santoscoy AV, Huntimer LM, Ramer-Tait AE, Wannemuehler M, Narasimhan B: Harvesting murine alveolar macrophages and evaluating cellular activation induced by polyanhydride nanoparticles. Journal of visualized experiments : JoVE 2012(64):e3883.

Biodegradable nanoparticles have emerged as a versatile platform for the design and implementation of new intranasal vaccines against […]

By |March 15th, 2017|Categories: Protocols, Publications, Respiratory System||0 Comments

Airway Hyperresponsiveness in Murine Tracheal Rings

Assessment of Airway Hyperresponsiveness in Murine Tracheal Rings. Methods Mol Biol. 2013;1032:257-69. doi: 10.1007/978-1-62703-496-8_20.

Isolated tracheal rings have often been used to directly measure the contractile output of airway smooth muscle (ASM). Here, we describe the method for excising murine tracheas, mounting tracheal rings in organ baths, and measuring the isometric forces generated by the ASM when […]

By |February 12th, 2017|Categories: Protocols, Respiratory System||0 Comments

Comparison of cell counting methods

Comparison of cell counting methods in rodent pulmonary toxicity studies: automated and manual protocols and considerations for experimental design

Patti C. Zeidler-Erdely, James M. Antonini, Terence G. Meighan, Shih-Houng Young, Tracy J. Eye, Mary Ann Hammer & Aaron Erdely (2016) Comparison of cell counting methods in rodent pulmonary toxicity studies: automated and manual protocols and […]

Differential Effects of Rapamycin and Dexamethasone

Differential Effects of Rapamycin and Dexamethasone in Mouse Models of Established Allergic Asthma

Mushaben EM, Brandt EB, Hershey GK, Le Cras TD. Differential effects of rapamycin and dexamethasone in mouse models of established allergic asthma. PLoS One. 2013;8(1):e54426. doi: 10.1371/journal.pone.0054426. Epub 2013 Jan 17.

The mammalian target of rapamycin (mTOR) plays an important role in cell […]

By |October 29th, 2014|Categories: Protocols, Publications, Respiratory System||0 Comments

Bilateral Vagotomy to Determine Autonomic Involvement in Airway Response

Jaime, Cyphert M. “Bilateral Vagotomy as a Tool for Determining Autonomic Involvement in Airway Responses in Mouse Models of Asthma” Springer Link, 2013. Web. 30 July 2014.

This chapter describes the use of bilateral vagotomy as a tool for determining autonomic regulation of airway responses to the exogenous bronchoconstrictor thromboxane mimetic U46619 in an acute model of […]

By |July 9th, 2014|Categories: Protocols, Respiratory System||0 Comments

PAMPs and DAMPs in Allergy Exacerbation Models

Willart, MA, P. Poulliot, BN Lambrecht, and M. Kool. “PAMPs and DAMPs in Allergy Exacerbation Models.” National Center for Biotechnology Information. U.S. National Library of Medicine, 2013. Web. 30 July 2014.

Sensitization of mice to real-life allergens or harmless antigen with the use of adjuvants will lead to the induction of DAMPs in the immune system. […]

By |July 9th, 2014|Categories: Respiratory System||0 Comments

Intranasal Influenza Infection of Mice

Sanders, CJ, B. Johnson, CW Frevert, and PG Thomas. “Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome.” National Center for Biotechnology Information. U.S. National Library of Medicine, 2013. Web. 30 July 2014.
Methods Mol Biol. 2013;1031:177-88. doi: 10.1007/978-1-62703-481-4_20.

In vivo influenza infection models are critical for understanding viral dynamics and host responses […]

By |July 9th, 2014|Categories: Protocols, Respiratory System||0 Comments

Flow Cytometric Methods to Assess Allergic Disease

Rahman, Adeeb H. “Flow Cytometric Methods for the Assessment of Allergic Disease – Springer.” Springer Link, 10 June 2013. Web. 30 July 2014.

Multiparametric flow cytometry is a powerful technique that allows the quantification and characterization of heterogeneous populations of cells. Advances in fl ow cytometric instrumentation, software, and reagents are occurring at a rapid pace, and […]

By |July 9th, 2014|Categories: Protocols, Respiratory System||0 Comments

Fibrocytes and Myofibroblasts in Allergic Asthma

Schmidt, Matthoias, and Sabrina Mattoli. “SpringerProtocols: Abstract: A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma.” Springer Protocols, 2013. Web. 30 July 2014.

Airway remodeling is a term used to collectively indicate bronchial structural changes that may lead to irreversible airflow obstruction and progressive decline in lung function […]

By |July 9th, 2014|Categories: Protocols, Respiratory System||0 Comments

Expression Profiling Associated with Allergic Phenotypes

Brickey, Willie June. “SpringerProtocols: Abstract: Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes.” Springer Protocols, 2013. Web. 30 July 2014.

Transcript profi ling reveals valuable insights to molecular and cellular activity related to disease. Gene expression profiles provide clues as to how tissues or cells in a particular environment may respond to stimuli. Gene-targeted examination […]

By |July 9th, 2014|Categories: Protocols, Respiratory System||0 Comments