Macpherson and McCoy Laboratories

Digestion medium: RPMI 2% FBS 1mg/ml collagenase et 0.1 mg/ml DNase

  • RPMI 1140 (GIMCO-ref 31870-025)
  • FBS-Foetal Bovine Serum (GIMO ref 10270-106)
  • Collagenase Type IA (Sigma C9891-1G)
  • DNase I (Roche 11 284 932 001)

Stop medium: RPMI 2% FBS 2mM EDTA

  • EDTA 0.5M ph 8.0 (GIMCO 15575-038)

Complete medium: RPMI 10% FBS 10mM HEPES 0.05 mM 2-mercaptoethanol 2mM Glutamine, 1% Penicillin-Streptomycin

  • HEPES 1M (GIMCO 15140-122)
  • 2-mercaptoethanol 50mM (GIMCO 31350-010)
  • Glutamine 200mM (Invitrogen 25030123)
  • Penicillin-Steptomycin (GIMCO) 15140-122)

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