Highly multiplexed simultaneous detection of RNAs and proteins in single cells

Andreas P Frei, Felice-Alessio Bava, Eli R Zunder, Elena W Y Hsieh, Shih-Yu Chen, Garry P Nolan & Pier Federico Gherardini: Nature Methods 2016 Jan; 269-275. Published online 2016 January 25. doi:10.1038/nmeth.3742.

This method combines the Flow cytometry possibility to acquire single cell expression of a defined marker with gene expression detection by fluorescence. Simultaneous single-celle detection of RNAs and protein may become more and more popular in the next future.

Read the full protocol here.