Isolation of T Cells from the Gut

Sonja Reissig , Christopher Hackenbruch , and Nadine Hövelmeyer. Isolation of T Cells from the Gut. DOI 10.1007/978-1-4939-1212-4_3,  Springer Science+Business Media New York 2014. 

The lymphocytes of epithelial and lamina proprial compartments of the intestine are phenotypically and functionally distinct and serve a wide range of functions in the intestinal mucosa like regulating intestinal homeostasis, maintaining epithelial barrier function as well as regulating adaptive and innate immune responses. To analyze the role of these cells in different disease states, it is necessary to isolate pure cell populations of the intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) of the gut. In this protocol we describe a method to isolate T cells from IEL and LPL, which can be used for further
investigations like comparative studies of mRNA expression, cell proliferation assay, or protein analysis.

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