Protocols

Comparison of cell counting methods

Comparison of cell counting methods in rodent pulmonary toxicity studies: automated and manual protocols and considerations for experimental design

Patti C. Zeidler-Erdely, James M. Antonini, Terence G. Meighan, Shih-Houng Young, Tracy J. Eye, Mary Ann Hammer & Aaron Erdely (2016) Comparison of cell counting methods in rodent pulmonary toxicity studies: automated and manual protocols and […]

By |November 4th, 2016|Categories: Protocols, Publications, Respiratory System|Tags: |0 Comments

In vivo Imaging and Tracking of Host-Microbiota Interactions via Metabolic

In vivo Imaging and Tracking of Host-Microbiota Interactions via Metabolic Labeling of Gut Anaerobic Bacteria
Naama Geva-Zatorsky, David Alvarez, Jason E. Hudak, Nicola C. Reading, Deniz Erturk-Hasdemir, Suryasarathi Dasgupta, Ulrich H. von Andrian, and Dennis L. Kasper: Nat Med. 2015 Sep; 21(9): 1091–1100. Published online 2015 Aug 17. doi:  10.1038/nm.3929

“The intestine is densely populated by anaerobic […]

T Lymphocyte Density and Distribution in Human Colorectal Mucosa, and Inefficiency of Current Cell Isolation Protocols

T Lymphocyte Density and Distribution in Human Colorectal Mucosa, and Inefficiency of Current Cell Isolation Protocols
Preza GC, Yang OO, Elliott J, Anton PA, Ochoa MT: T Lymphocyte Density and Distribution in Human Colorectal Mucosa, and Inefficiency of Current Cell Isolation Protocols. PLoS One 2015 Apr 9;10(4):e0122723.

“Mucosal tissues are critical immune effector sites containing complex populations of […]

By |February 19th, 2016|Categories: Gastrointestinal System, Protocols, Publications|Tags: |0 Comments

Isolation and Cytokine Analysis of Lamina Propria Lymphocytes

Isolation and Cytokine Analysis of Lamina Propria Lymphocytes from Mucosal Biopsies of the Human Colon
Bowcutt R, Malter LB, Chen LA, Wolff MJ, Robertson I, Rifkin DB, Poles M, Cho I, Loke P: Isolation and Cytokine Analysis of Lamina Propria Lymphocytes from Mucosal Biopsies of the Human Colon. J Immunol Methods 2015 Jun;421:27-35. 

“Much of our understanding of […]

By |February 19th, 2016|Categories: Gastrointestinal System, Protocols, Publications|Tags: |0 Comments

Defining Human Dendritic Cell Progenitors by Multiparametric Flow Cytometry

Defining Human Dendritic Cell Progenitors by Multiparametric Flow Cytometry

G. Breton, J. Lee, K. Liu, M.C. Nussenzweig: Defining human dendritic cell progenitors by multiparametric flow cytometry. Nat Protoc. 2015 Sep;10(9):1407-22. PMC4607256.

Dendritic cells (DCs) play an important role in the maintenance of mucosal homeostasis and the induction of antigen-specific mucosal immune responses.  DCs develop from bone marrow from increasingly restricted but phenotypically well-defined progenitor […]

By |November 30th, 2015|Categories: 2015, News, Protocols, Publications, SMI Archives|Tags: |0 Comments

Generating Human Intestinal Tissue from Pluripotent Stem Cells

Generating Human Intestinal Tissue from Pluripotent Stem Cells In Vitro

K.W. McCracken, J.C. Howell, J.M. Wells, J.R. Spence:  Generating human intestinal tissue from pluripotent stem cells in vitro.  Nat Protoc. 2011 Nov 10;6(12):1920-8. PMC3896236

Three-dimensional cultures of mucosal epithelial cells termed organoids are considered the most physiological models of primary epithelial cells in vitro and have […]

By |November 30th, 2015|Categories: 2015, News, Protocols, Publications, SMI Archives|Tags: |0 Comments

Advanced CUBIC protocols for whole-brain and

Advanced CUBIC Protocols for Whole-Brain and Whole-Body Clearing and Imaging

Etsuo A Susaki, Kazuki Tainaka, Dimitri Perrin, Hiroko Yukinaga, Akihiro Kuno and Hiroki R Ueda: Advanced CUBIC Protocols for Whole-Brain and Whole-Body Clearing and Imaging. Nature protocols 2015, 10: 1709-1727.

This protocol describes in detail how to perform advanced CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis). The CUBIC protocol enables simple and […]

By |October 26th, 2015|Categories: Laboratory Techniques, Protocols, Publications|Tags: |0 Comments

Models of Oral and Vaginal Candidiasis based on in vitro

Models of Oral and Vaginal Candidiasis Based on in vitro Reconstituted Human Epithelia

Schaller M, Zakikhany K, Naglik JR, Weindl G, Hube B: Models of oral and vaginal candidiasis based on in vitro reconstituted human epithelia. Nature protocols 2006, 1(6):2767-2773.

This protocol describes the setup, maintenance and characteristics of models of epithelial Candida infections based on […]

By |October 29th, 2014|Categories: Genitourinary Tract, Protocols, Publications|Tags: |0 Comments

Analysis of Murine Lamina Propria Mononuclear Cells

Isolation and Subsequent Analysis of Murine Lamina Propria Mononuclear Cells from Colonic Tissue

Weigmann B, Tubbe I, Seidel D, Nicolaev A, Becker C, Neurath MF: Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue. Nature protocols 2007, 2(10):2307-2311.

Studies on colonic cells in the lamina propria (LP) of mine are important for […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications|Tags: |0 Comments

Optimization of Murine Small Intestine Leukocyte Isolation

Optimization of Murine Small Intestine Leukocyte Isolation for Global Immune Phenotype Analysis

Andrew W. Goodyear, Ajay Kumar, Steven Dow, Elizabeth P. Ryan. Optimization of murine small intestine leukocyte isolation for global immune phenotype analysis. Journal of Immunological Methods 405 (2014) 97–108.

New efforts to understand complex interactions between diet, gut microbiota, and intestinal immunity emphasize the […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications|Tags: |0 Comments