Protocols

Immunological Changes in IL-10-Deficient Mice

New Insights into the Immunological Changes in IL-10-Deficient Mice during the Course of Spontaneous Inflammation in the Gut Mucosa

Gomes-Santos AC, Moreira TG, Castro-Junior AB, Horta BC, Lemos L, Cruz DN, Guimarães MA, Cara DC, McCafferty DM, Faria AM. New insights into the immunological changes in IL-10-deficient mice during the course of spontaneous inflammation in […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications|Tags: |0 Comments

Differential Effects of Rapamycin and Dexamethasone

Differential Effects of Rapamycin and Dexamethasone in Mouse Models of Established Allergic Asthma

Mushaben EM, Brandt EB, Hershey GK, Le Cras TD. Differential effects of rapamycin and dexamethasone in mouse models of established allergic asthma. PLoS One. 2013;8(1):e54426. doi: 10.1371/journal.pone.0054426. Epub 2013 Jan 17.

The mammalian target of rapamycin (mTOR) plays an important role in cell […]

By |October 29th, 2014|Categories: Protocols, Publications, Respiratory System|Tags: |0 Comments

Oral Delivery of Protein Complement Inhibitor

A Novel Protocol Allowing Oral Delivery of a Protein Complement Inhibitor that Subsequently Targets to Inflamed Colon Mucosa and Ameliorates Murine Colitis

Elvington, M; Blichmann, P; Qiao, F; Scheiber, M; Wadsworth, C; et al. A novel protocol allowing oral delivery of a protein complement inhibitor that subsequently targets to inflamed colon mucosa and ameliorates murine […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications|Tags: |0 Comments

Isolation of T Cells from the Gut

Isolation of T Cells from the Gut

Sonja Reissig , Christopher Hackenbruch , and Nadine Hövelmeyer. Isolation of T Cells from the Gut. DOI 10.1007/978-1-4939-1212-4_3,  Springer Science+Business Media New York 2014. 

The lymphocytes of epithelial and lamina proprial compartments of the intestine are phenotypically and functionally distinct and serve a wide range of functions in the […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications|Tags: |0 Comments

Confocal Laser Endomicroscopy for in vivo Imaging

Confocal Laser Endomicroscopy and Narrow-band Imaging-aided Endoscopy for in vivo Imaging of Colitis and Colon Cancer in Mice

Waldner MJ, Wirtz S, Neufert C, Becker C, Neurath MF: Confocal laser endomicroscopy and narrow-band imaging-aided endoscopy for in vivo imaging of colitis and colon cancer in mice. Nature protocols 2011, 6(9):1471-1481.

New endoscopic techniques such as narrow-band […]

By |October 29th, 2014|Categories: Gastrointestinal System, Protocols, Publications|Tags: |0 Comments

Compression-Based Distance (CBD): Microbiota Composition

Fang, Y., Chia, N., White, B., Schook, L. “Compression-based Distance (CBD): A Simple, Rapid, and Accurate Method for Microbiota Composition Comparison.” BMC Bioinformatics. BMC Bioinformatics, 23 Apr. 2013.

Perturbations in intestinal microbiota composition have been associated with a variety of gastrointestinal tract-related diseases. The alleviation of symptoms has been achieved using treatments that alter the gastrointestinal tract […]

By |July 25th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

Incubation of Precision-Cut Liver and Intestinal Slices

Preparation and Incubation of Precision-cut Liver and Intestinal Slices for Application in Drug Metabolism. de Graaf IA, Olinga P, de Jager MH, Merema MT, de Kanter R, van de Kerkhof EG, Groothuis GM.  Nat Protoc. 2010 Sep;5(9):1540-51. doi: 10.1038/nprot.2010.111. Epub 2010 Aug 19.

Precision-cut tissue slices (PCTS) are viable ex vivo explants of tissue with a […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

Methods for Generating and Colonizing Gnotobiotic Zebrafish

Pham LN, Kanther M, Semova I, Rawls JF.  Nat Protoc. 2008;3(12):1862-75. doi: 10.1038/nprot.2008.186.

Vertebrates are colonized at birth by complex and dynamic communities of microorganisms that can contribute significantly to host health and disease. The ability to raise animals in the absence of microorganisms has been a powerful tool for elucidating the relationships between animal […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

Chemically Induced Mouse Models of Intestinal Inflammation

Wirtz S, Neufert C, Weigmann B, Neurath MF.  Nat Protoc. 2007;2(3):541-6.

Animal models of intestinal inflammation are indispensable for our understanding of the pathogenesis of Crohn’s disease and ulcerative colitis, the two major forms of inflammatory bowel disease in humans. Here, we provide protocols for establishing murine 2,4,6-trinitro benzene sulfonic acid (TNBS)-, oxazolone- and both […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

High Resolution Colonoscopy in Live Mice

Becker C, Fantini MC, Neurath MF. Nat Protoc. 2006;1(6):2900-4.

Endoscopy in humans is a powerful method for physicians to examine the gut for inflammatory or neoplastic changes. In medical and immunological research, animal models of intestinal diseases are established key tools to investigate the mucosal immune system, colitis and cancer development in the gut. Moreover, […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments