Protocols

High Resolution Colonoscopy in Live Mice

Becker C, Fantini MC, Neurath MF. Nat Protoc. 2006;1(6):2900-4.

Endoscopy in humans is a powerful method for physicians to examine the gut for inflammatory or neoplastic changes. In medical and immunological research, animal models of intestinal diseases are established key tools to investigate the mucosal immune system, colitis and cancer development in the gut. Moreover, […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

Isolating, Immunophenotyping and ex vivo Stimulation

Isolating, Immunophenotyping and ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes during Murine Helicobacter pylori Infection. Victoria E. Ruiz, Monisha Sachdev, Songhua Zhang, Sicheng Wen, Steven F. Moss.  Journal of Immunological Methods, Volume 384, Issues 1–2, 31 October 2012, Pages 157-163

Helicobacter pylori infection is associated with severe chronic inflammation, yet the host immune response is rarely […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

Niche-Independent High-Purity Cultures of Lgr5+

Niche-Independent High-Purity Cultures of Lgr5+ Intestinal Stem Cells and their Progeny. Yin X, Farin HF, van Es JH, Clevers H, Langer R, Karp JM. Nat Methods. 2014 Jan;11(1):106-12. doi: 10.1038/nmeth.2737. Epub 2013 Dec 1.

Although Lgr5+ intestinal stem cells have been expanded in vitro as organoids, homogeneous culture of these cells has not been possible thus […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

Bacterial Mediated Gastrointestinal Inflammation

Uronis J, Sun X. Methods Mol Biol. 2013;1031:197-202. doi: 10.1007/978-1-62703-481-4_22.

Mouse models have proven to be a key approach in our understanding of the etiology and physiology underlying bacterial mediated gastrointestinal infl ammation. Generally, these models are based on the inoculation of genetically susceptible mice with either commensal or pathogenic bacteria to elicit an inflammatory response. Here, we […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

An Inducible Mouse Model of Colon Carcinogenesis

An Inducible Mouse Model of Colon Carcinogenesis for the Analysis of Sporadic and Inflammation-Driven Tumor Progression. Neufert C, Becker C, Neurath MF. Nat Protoc. 2007;2(8):1998-2004.

Colorectal cancer is a life-threatening disease that can develop spontaneously or as a complication of inflammatory bowel diseases. Mouse models are essential tools for the preclinical testing of novel therapeutic […]

By |July 24th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

Bilateral Vagotomy to Determine Autonomic Involvement in Airway Response

Jaime, Cyphert M. “Bilateral Vagotomy as a Tool for Determining Autonomic Involvement in Airway Responses in Mouse Models of Asthma” Springer Link, 2013. Web. 30 July 2014.

This chapter describes the use of bilateral vagotomy as a tool for determining autonomic regulation of airway responses to the exogenous bronchoconstrictor thromboxane mimetic U46619 in an acute model of […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

PAMPs and DAMPs in Allergy Exacerbation Models

Willart, MA, P. Poulliot, BN Lambrecht, and M. Kool. “PAMPs and DAMPs in Allergy Exacerbation Models.” National Center for Biotechnology Information. U.S. National Library of Medicine, 2013. Web. 30 July 2014.

Sensitization of mice to real-life allergens or harmless antigen with the use of adjuvants will lead to the induction of DAMPs in the immune system. […]

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Intranasal Influenza Infection of Mice

Sanders, CJ, B. Johnson, CW Frevert, and PG Thomas. “Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome.” National Center for Biotechnology Information. U.S. National Library of Medicine, 2013. Web. 30 July 2014.
Methods Mol Biol. 2013;1031:177-88. doi: 10.1007/978-1-62703-481-4_20.

In vivo influenza infection models are critical for understanding viral dynamics and host responses […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Flow Cytometric Methods to Assess Allergic Disease

Rahman, Adeeb H. “Flow Cytometric Methods for the Assessment of Allergic Disease – Springer.” Springer Link, 10 June 2013. Web. 30 July 2014.

Multiparametric flow cytometry is a powerful technique that allows the quantification and characterization of heterogeneous populations of cells. Advances in fl ow cytometric instrumentation, software, and reagents are occurring at a rapid pace, and […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Fibrocytes and Myofibroblasts in Allergic Asthma

Schmidt, Matthoias, and Sabrina Mattoli. “SpringerProtocols: Abstract: A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma.” Springer Protocols, 2013. Web. 30 July 2014.

Airway remodeling is a term used to collectively indicate bronchial structural changes that may lead to irreversible airflow obstruction and progressive decline in lung function […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments