Protocols

Cockroach Antigen Model of Acute Asthma

Durham, Carolyn G., Lisa M. Schwiebert, and Robin G. Lorenz. “Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection – Springer.” Springer Link, 10 June 2013. Web. 30 July 2014.

The increased incidence of asthma over the last 50 years in developed countries has […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Clara Epithelial Cell Depletion in the Lung

Sonar, Sanchaita S., and Jan C. Dudda. “Clara Epithelial Cell Depletion in the Lung – Springer.” Springer Link, 10 June 2013. Web. 30 July 2014.

The bronchial epithelium has been increasingly recognized as an important immunomodulatory compartment in asthma and other lung diseases. Clara cells, which comprise the nonciliated secretory epithelial cells, are an important epithelial cell […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Bronchoscopic Imaging of Pulmonary Responses

Bronchoscopic Imaging of Pulmonary Mucosal Vasculature Responses to Inflammatory Mediators. J Biomed Opt. 2005 May-Jun;10(3):034013.

In current clinical practice, the initial stage in the detection of pulmonary airway disease is the clinician’s interpretation of a bronchoscopy procedure. Due to the nature of this form of detection, diseases are often well advanced upon discovery. This paper proposes a new method […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Inhalation Model of Allergic Fungal Asthma

An Inhalation Model of Airway Allergic Response to Inhalation of Environmental Aspergillus Fumigatus Conidia in Sensitized BALB/c Mice. Med Mycol. 2010 Dec;48(8):1056-65. doi: 10.3109/13693786.2010.485582. Epub 2010 May 20.

The ability to accurately mimic normal processes for sensitization and allergen challenge in an experimental animal model are useful in that they allow researchers to critically manipulate the […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Induction of Allergic Rhinitis in Mice

Methods Mol Biol. 2013;1032:145-58. doi: 10.1007/978-1-62703-496-8_12.

We describe a method for allergic rhinitis (AR) induction in mice. Methodology involves nasal infusions of small volumes of ovalbumin for both initial sensitization and challenges. The latter are frequent and carried out over several weeks. This methodology more closely resembles natural AR induction than does the common use of systemic sensitization, […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Induction and Evaluation of Systemic Anaphylaxis in Mice

Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice. Methods Mol Biol. 2013;1032:133-8. doi: 10.1007/978-1-62703-496-8_10.

Mouse models of systemic anaphylaxis are important tools for the study of mast cell function, for the elucidation of the pathomechanisms of anaphylaxis, and for identifying and characterizing potential therapies for anaphylaxis. Here, we describe two murine models of systemic […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Assessment of Airway Hyperresponsiveness in Mouse Models

Hartney, John M; Robichaud, Annette. Assessment of Airway Hyperresponsiveness in Mouse Models of Allergic Lung Disease Using Detailed Measurements of Respiratory Mechanics. Methods Mol Biol. 2013;1032:205-17. doi: 10.1007/978-1-62703-496-8_16.

This chapter provides an outline of the procedures necessary to measure airway hyperresponsiveness to inhaled methacholine in mouse models of allergic lung disease. We present a method for […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Bacteria-Mediated Acute Lung Inflammation

Allen, Irving C. Bacteria-Mediated Acute Lung Inflammation. Methods Mol Biol. 2013;1031:163-75. doi: 10.1007/978-1-62703-481-4_19.

Mouse models of acute lung inflammation are critical for understanding the role of the innate immune response to pathogen associated molecular patterns, bacteria, and sepsis in humans. Bacterial infections in the lung elicit a range of immune reactions, depending on the pathogen, the level of […]

By |July 9th, 2014|Categories: Protocols, Respiratory System|Tags: |0 Comments

Evaluation of T Cell Function in Allergic Disease

Barker, B. Methods Mol Biol. 2013;1032:31-44. doi: 10.1007/978-1-62703-496-8_3.

T lymphocytes play positive and negative roles in the pathogenesis of allergic disease. Isolation and functional characterization of T lymphocyte subpopulations is an important aspect of understanding allergy models and allergy therapies. Measurement of the T cell surface proteins and T cell proliferation can provide insight into T cell activation. […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Assessment of Oxidative Metabolism

Imbeault E, Gris D. Methods Mol Biol. 2013;1031:51-7. doi: 10.1007/978-1-62703-481-4_6.

Oxidative metabolism is one of the central physiological processes that regulate multiple functions in a cell including cell death and survival, proliferation, gene transcription, and protein modification. There are multitudes of techniques that are used to evaluate oxidative activity. Here, we summarize how to measure oxidative activity by […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments