Protocols

Evaluating B Cells: Bone Marrow Precursors

Evaluating B-Cells: From Bone Marrow Precursors to Antibody-Producing Cells. Rosado MM, Scarsella M, Cascioli S, Giorda E, Carsetti R. Methods Mol Biol. 2013;1032:45-57. doi: 10.1007/978-1-62703-496-8_4.

Lymphocyte characterization is primarily based on the differential expression of surface markers. In this context, flow-cytometry analysis (FACS) is an exceptional technique that not only allows the identification of B-cell subsets, but can […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture

Nakano H, Cook DN. Methods Mol Biol. 2013;1032:19-29. doi: 10.1007/978-1-62703-496-8_2.

Antigen presenting cells (APCs) such as dendritic cells (DCs) and macrophages comprise a relatively small fraction of leukocytes residing in lymphoid and non-lymphoid tissues. Accordingly, functional analyses of these cells have been hampered by low cell yields. Also, alveolar macrophages share several physical properties with DCs, and this […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Applications of Mouse Airway Epithelial Cell Culture for Asthma Research

Applications of Mouse Airway Epithelial Cell Culture for Asthma Research. Horani A, Dickinson JD, Brody SL. Methods Mol Biol. 2013;1032:91-107. doi: 10.1007/978-1-62703-496-8_7.

Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell–cell interactions, and the role of immune system factors in asthma pathogenesis. In this chapter, we discuss the application of mouse tracheal […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Orogastrointestinal Model of Nucosal Candidiasis

Orogastrointestinal Model of Nucosal and Disseminated Candidiasis. Clemons KV, Stevens DA.  Methods Mol Biol. 2012;845:557-67. doi: 10.1007/978-1-61779-539-8_41.

Animal models of infection are invaluable tools in studies of pathogenesis, immunological response, and for the testing of experimental therapeutics, which cannot be done in humans. Murine models of infection are used most often for these studies and […]

By |June 20th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

An Introduction to Saccharomyces Cerevisia

JoVE Science Education Database. Model Organisms I: yeast, Drosophila and C. elegans. An Introduction to Saccharomyces cerevisiae. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5081 (2014).

Saccharomyces cerevisiae (commonly known as baker’s yeast) is a single-celled eukaryote that is frequently used in scientific research. S. cerevisiae is an attractive model organism due to the fact […]

By |June 3rd, 2014|Categories: Laboratory Techniques|Tags: |0 Comments

An Introduction to the Laboratory Mouse: Mus Musculus

JoVE Science Education Database. Model Organisms II: Mouse, Zebrafish, and Chick. An Introduction to the Laboratory Mouse: Mus musculus. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5129 (2014).

Mice (Mus musculus) are an important research tool for modeling human disease progression and development in the lab. Despite differences in their size and appearance, mice share […]

By |June 3rd, 2014|Categories: Laboratory Techniques|Tags: |0 Comments

An Introduction to the Zebrafish: Danio Rerio

JoVE Science Education Database. Model Organisms II: Mouse, Zebrafish, and Chick. An Introduction to the Zebrafish: Danio rerio. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5128 (2014).

Zebrafish (Danio rerio) are small freshwater fish that are used as model organisms for biomedical research. The many strengths of these fish include their high degree of genetic […]

By |June 3rd, 2014|Categories: Laboratory Techniques|Tags: |0 Comments

An Introduction to Transfection

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. An Introduction to Transfection. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5068 (2014).

Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA enables the expression, or production, of proteins using the […]

By |June 3rd, 2014|Categories: Laboratory Techniques|Tags: |0 Comments

An Introduction to Working in the Hood

JoVE Science Education Database. General Laboratory Techniques. An Introduction to Working in the Hood. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5036 (2014).

Hoods are a common class of instrument, which act as a protective enclosure for various types of laboratory experiments. They serve to shield experimental samples from the environment, as well as to […]

By |June 3rd, 2014|Categories: Laboratory Techniques|Tags: |0 Comments

An Introduction to the Chick: Gallus Gallus Domesticus

JoVE Science Education Database. Model Organisms II: Mouse, Zebrafish, and Chick. An Introduction to the Chick: Gallus gallus domesticus. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5153 (2014).

The chicken embryo (Gallus gallus domesticus) is an extremely valuable model organism for research in developmental biology, in part because most of their development takes place within […]

By |June 3rd, 2014|Categories: Laboratory Techniques|Tags: |0 Comments