Protocols

Quantification of Neturophil Extracellular Traps

Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils. Vong L, Sherman PM, Glogauer M. Methods Mol Biol. 2013;1031:41-50. doi: 10.1007/978-1-62703-481-4_5.

Neutrophils are one of the first cells to respond to an inflammatory stimulus, and are equipped with an assortment of antimicrobial and proteolytic enzymes to disarm and degrade bacterial pathogens. A novel mechanism of […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Mast Cells in Mouse Models of Allergic Disease

Isolation and Characterization of Mast Cells in Mouse Models of Allergic Diseases. Kovarova, M. Methods Mol Biol. 2013;1032:109-19. doi: 10.1007/978-1-62703-496-8_8.

After their activation, mast cells release a variety of bioactive mediators that contribute to characteristic symptoms of allergic reactions. Ex vivo analysis of mast cells derived from their progenitors or isolated from mice is an indispensable tool for the […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Protocols for Enumerating Mouse Eosinophils

Dyer KD, Garcia-Crespo KE, Percopo CM, Sturm EM, Rosenberg HF. Methods Mol Biol. 2013;1032:59-77. doi: 10.1007/978-1-62703-496-8_5.

Eosinophils are prominent in allergic diseases, and their effector functions are studied in numerous gene-deleted and transgenic mouse models. However, mouse eosinophils and human eosinophils are not structurally or functionally equivalent, and assays designed to evaluate the properties of human eosinophils may […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

DC Isolation from Lymph Node

Macpherson and McCoy Laboratories

Digestion medium: RPMI 2% FBS 1mg/ml collagenase et 0.1 mg/ml DNase

RPMI 1140 (GIMCO-ref 31870-025)
FBS-Foetal Bovine Serum (GIMO ref 10270-106)
Collagenase Type IA (Sigma C9891-1G)
DNase I (Roche 11 284 932 001)

Stop medium: RPMI 2% FBS 2mM EDTA

EDTA 0.5M ph 8.0 (GIMCO 15575-038)

Complete medium: RPMI 10% FBS 10mM HEPES 0.05 mM 2-mercaptoethanol 2mM Glutamine, […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Regulatory Functions of Bone Marrow-Derived Macrophages

Evaluation of Classical, Alternative, and Regulatory Functions of Bone Marrow-Derived Macrophages. Davis BK. Methods Mol Biol. 2013;1032:79-89. doi: 10.1007/978-1-62703-496-8_6.

The role of macrophage subsets in allergic diseases in vivo is under current investigation. These cells perform sentinel functions in the lung, the skin, and the gastrointestinal mucosa. Their interface with environmental cues influences the initiation, progression, development, and resolution […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Evaluating B Cells: Bone Marrow Precursors

Evaluating B-Cells: From Bone Marrow Precursors to Antibody-Producing Cells. Rosado MM, Scarsella M, Cascioli S, Giorda E, Carsetti R. Methods Mol Biol. 2013;1032:45-57. doi: 10.1007/978-1-62703-496-8_4.

Lymphocyte characterization is primarily based on the differential expression of surface markers. In this context, flow-cytometry analysis (FACS) is an exceptional technique that not only allows the identification of B-cell subsets, but can […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture

Nakano H, Cook DN. Methods Mol Biol. 2013;1032:19-29. doi: 10.1007/978-1-62703-496-8_2.

Antigen presenting cells (APCs) such as dendritic cells (DCs) and macrophages comprise a relatively small fraction of leukocytes residing in lymphoid and non-lymphoid tissues. Accordingly, functional analyses of these cells have been hampered by low cell yields. Also, alveolar macrophages share several physical properties with DCs, and this […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Applications of Mouse Airway Epithelial Cell Culture for Asthma Research

Applications of Mouse Airway Epithelial Cell Culture for Asthma Research. Horani A, Dickinson JD, Brody SL. Methods Mol Biol. 2013;1032:91-107. doi: 10.1007/978-1-62703-496-8_7.

Primary airway epithelial cell culture provides a valuable tool for studying cell differentiation, cell–cell interactions, and the role of immune system factors in asthma pathogenesis. In this chapter, we discuss the application of mouse tracheal […]

By |July 8th, 2014|Categories: Ex Vivo, Protocols|Tags: |0 Comments

Orogastrointestinal Model of Nucosal Candidiasis

Orogastrointestinal Model of Nucosal and Disseminated Candidiasis. Clemons KV, Stevens DA.  Methods Mol Biol. 2012;845:557-67. doi: 10.1007/978-1-61779-539-8_41.

Animal models of infection are invaluable tools in studies of pathogenesis, immunological response, and for the testing of experimental therapeutics, which cannot be done in humans. Murine models of infection are used most often for these studies and […]

By |June 20th, 2014|Categories: Gastrointestinal System, Protocols|Tags: |0 Comments

An Introduction to Saccharomyces Cerevisia

JoVE Science Education Database. Model Organisms I: yeast, Drosophila and C. elegans. An Introduction to Saccharomyces cerevisiae. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5081 (2014).

Saccharomyces cerevisiae (commonly known as baker’s yeast) is a single-celled eukaryote that is frequently used in scientific research. S. cerevisiae is an attractive model organism due to the fact […]

By |June 3rd, 2014|Categories: Laboratory Techniques|Tags: |0 Comments