Protocols

An Introduction to the Laboratory Mouse: Mus Musculus

JoVE Science Education Database. Model Organisms II: Mouse, Zebrafish, and Chick. An Introduction to the Laboratory Mouse: Mus musculus. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5129 (2014).

Mice (Mus musculus) are an important research tool for modeling human disease progression and development in the lab. Despite differences in their size and appearance, mice share […]

By |June 3rd, 2014|Categories: Laboratory Techniques|Tags: |0 Comments

An Introduction to the Zebrafish: Danio Rerio

JoVE Science Education Database. Model Organisms II: Mouse, Zebrafish, and Chick. An Introduction to the Zebrafish: Danio rerio. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5128 (2014).

Zebrafish (Danio rerio) are small freshwater fish that are used as model organisms for biomedical research. The many strengths of these fish include their high degree of genetic […]

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An Introduction to Transfection

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. An Introduction to Transfection. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5068 (2014).

Transfection is the process of inserting genetic material, such as DNA and double stranded RNA, into mammalian cells. The insertion of DNA enables the expression, or production, of proteins using the […]

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An Introduction to Working in the Hood

JoVE Science Education Database. General Laboratory Techniques. An Introduction to Working in the Hood. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5036 (2014).

Hoods are a common class of instrument, which act as a protective enclosure for various types of laboratory experiments. They serve to shield experimental samples from the environment, as well as to […]

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An Introduction to the Chick: Gallus Gallus Domesticus

JoVE Science Education Database. Model Organisms II: Mouse, Zebrafish, and Chick. An Introduction to the Chick: Gallus gallus domesticus. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5153 (2014).

The chicken embryo (Gallus gallus domesticus) is an extremely valuable model organism for research in developmental biology, in part because most of their development takes place within […]

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An Overview of the Model Organism: C. Elegans

JoVE Science Education Database. Model Organisms I: yeast, Drosophila and C. elegans. An Overview of the Model Organism: C. elegans. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5103 (2014).

Caenorhabditis elegans is a microscopic, soil-dwelling roundworm that has been powerfully used as a model organism since the early 1970″s. It was initially proposed as a […]

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Bacterial Transformation: Electroporation

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Bacterial Transformation: Electroporation. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5060 (2014).

The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores […]

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DNA Ligation Reactions

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. DNA Ligation Reactions. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5069 (2014).

Overview
Ligation can be defined as the act of joining, and in biology the term refers to an enzymatic reaction that joins two biomolecules with a covalent bond. This video describes the application […]

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Gel Purification

JoVE Science Education Database. Basic Methods in Cellular and Molecular Biology. Gel Purification. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5063 (2014).

Overview
Gel-purification is a standard procedure performed to recover desired DNA fragments from agarose gels after electrophoretic separation. After dissolving the gel fragment and running it through a specialized filter, this procedure yields DNA […]

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Histological Sample Preparation for Light Microscopy

JoVE Science Education Database. General Laboratory Techniques. Histological Sample Preparation for Light Microscopy. Journal of Visualized Experiments, Cambridge, MA, doi: 10.3791/5039 (2014).

Histology is the study of cells and tissues, which is typically aided by the use of a light microscope. The preparation of histological samples can vary greatly based on the inherent properties of […]

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