Sequencing and cloning of antigen-specific antibodies from mouse memory B cells

Published in Nature Protocols

Lotta von Boehmer, Cassie Liu, Sarah Ackerman, Alexander D Gitlin, Qiao Wang, Anna Gazumyan & Michel C Nussenzweig :Nature Protocols 11, 1908-1923 (2016) doi:10.1038/nprot.2016.102, Published online 15 September 2016

Methods to identify genes encoding immunoglobulin heavy and light chains from single B lymphocytes vary in efficiency, error rate and practicability. Here we describe […]

High-resolution 3D analysis of mouse small-intestinal stroma

Published in Nature Protocols

Jeremiah Bernier-Latmani & Tatiana V Petrova :Nature Protocols 11, 1617–1629 (2016) doi:10.1038/nprot.2016.092, Published online 11 August 2016

Here we detail a protocol for whole-mount immunostaining of mouse small-intestinal villi that can be used to generate high-resolution 3D images of all gut cell types, including blood and lymphatic vessel cells, neurons, smooth muscle […]