Video Article Published on JoVE

Rothschild DE, Srinivasan T, Aponte-Santiago LA, Shen X, Allen IC : JoVE 111, (2016) doi:10.3791/54033, Published 18 May 2016

This protocol has been generated to outline a simple procedure to culture and treat small intestinal organoids with microbial pathogens and pathogen associated molecular patterns (PAMPs). It also emphasizes the normalization techniques that should be applied when protein analysis are conducted after such a challenge.

Link to JoVE: http://www.jove.com/video/54033/the-ex-vivo-culture-pattern-recognition-receptor-stimulation-mouse?status=a56039k

NIH Search Link: https://www.ncbi.nlm.nih.gov/pubmed/27285214