Quantification of Neturophil Extracellular Traps
Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils. Vong L, Sherman PM, Glogauer M. Methods Mol Biol. 2013;1031:41-50. doi: 10.1007/978-1-62703-481-4_5.
Neutrophils are one of the first cells to respond to an inflammatory stimulus, and are equipped with an assortment of antimicrobial and proteolytic enzymes to disarm and degrade bacterial pathogens. A novel mechanism of bacterial trapping, termed neutrophil extracellular traps (NETs), was recently described whereby neutrophils were shown to cast out web-like structures of chromatin, capturing and immobilizing invading pathogens. Herein we describe protocols to isolate murine bone marrow-derived neutrophils,and spectrophotometrically quantify, immunolabel, and visualize NET structures in vitro.