The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
1/11/2017
Rothschild DE, Srinivasan T, Aponte-Santiago LA, Shen X, Allen IC : JoVE 111, (2016) doi:10.3791/54033, Published 18 May 2016
This protocol has been generated to outline a simple procedure to culture and treat small intestinal organoids with microbial pathogens and pathogen associated molecular patterns (PAMPs). It also emphasizes the normalization techniques that should be applied when protein analysis are conducted after such a challenge.
